TPO Antibody
| Code | Size | Price |
|---|
| PSI-XP-5288-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
TPO antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th
Documents
Further Information
Additional Names:
ML, TPO, MGDF, MKCSF, MPLLG, THCYT1, Thrombopoietin, C-mpl ligand, ML
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5 ng/mL), a concentration of 4.2 - 6.3 μg/mL of this antibody is required.
ELISA:
To detect hTPO by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTPO.
Sandwich:
To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TPO (XP-5288Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5 ng/mL), a concentration of 4.2 - 6.3 μg/mL of this antibody is required.
ELISA:
To detect hTPO by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTPO.
Sandwich:
To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TPO (XP-5288Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO.
NCBI Gene ID #:
7066
NCBI Official Name:
thrombopoietin
NCBI Official Symbol:
THPO
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P40225
Protein GI Number:
730982
Purification:
Anti-hTPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.
Research Area:
Stem Cell,Antibody Pairs, Functional Assays
Swissprot #:
P40225
User NOte:
Centrifuge vial prior to opening.