Resistin Antibody
| Code | Size | Price |
|---|
| PSI-XP-5260-0.1mg | 0.1mg | £524.00 |
Quantity:
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Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Resistin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated free
Documents
Further Information
Additional Names:
ADSF, Rstn, Xcp4, Fizz3, Resistin, Adipose tissue-specific secretory factor, ADSF
Application Note:
ELISA:
To detect mResistin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mResistin.
Sandwich:
To detect mResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Resistin (XP-5260Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mResistin.
Western Blot:
To detect mResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mResistin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mResistin.
Sandwich:
To detect mResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Resistin (XP-5260Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mResistin.
Western Blot:
To detect mResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mResistin (murine Resistin).
NCBI Gene ID #:
57264
NCBI Official Name:
resistin
NCBI Official Symbol:
Retn
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q99P87
Protein GI Number:
17380301
Purification:
Anti-mResistin specific antibody was purified by affinity chromatography employing immobilized mResistin matrix.
Research Area:
Chemokines & Cytokines,Signal Transduction,Antibody Pairs
Swissprot #:
Q99P87
User NOte:
Centrifuge vial prior to opening.