IL-8 Antibody

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ProSci
Product Code: PSI-XP-5199
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5199-0.1mg0.1mg£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
IL-8 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t

Images

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To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-8.
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To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1.0 ug/ml for two hours at room temperature or 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
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To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-8.
To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1.0 ug/ml for two hours at room temperature or 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.

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Further Information

Additional Names:
NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, Interleukin-8, C-X-C motif chemokine 8, IL-8
Application Note:
ELISA:

To detect hIL-8 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-8.

Sandwich:

To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-8.

Western Blot:
To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-8 (human Interleukin-8).
NCBI Gene ID #:
3576
NCBI Official Name:
interleukin 8
NCBI Official Symbol:
IL8
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P10145
Protein GI Number:
124359
Purification:
Anti-hIL-8 specific antibody was purified by affinity chromatography employing immobilized hIL-8 matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P10145
User NOte:
Centrifuge vial prior to opening.