I-TAC Antibody

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ProSci
Product Code: PSI-XP-5205
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5205-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
I-TAC antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

Images

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To detect hI-TAC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human I-TAC (XP-5205Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-TAC.
2 / 5
To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 5
To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 5
This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.125ug/ml- 0.25ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
5 / 5
This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.125ug/ml- 0.25ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
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To detect hI-TAC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human I-TAC (XP-5205Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-TAC.
To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.125ug/ml- 0.25ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.125ug/ml- 0.25ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.

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Further Information

Additional Names:
IP9, H174, IP-9, b-R1, I-TAC, SCYB11, SCYB9B, ITAC, C-X-C motif chemokine 11, Beta-R1
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hI-TAC (100 ng/mL), a concentration of 2.0 - 3.0 μg/mL of this antibody is required.

ELISA:

To detect hI-TAC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hI-TAC.

Sandwich:

To detect hI-TAC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human I-TAC (XP-5205Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-TAC.

Western Blot:

To detect hI-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-TAC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hI-TAC (human Interferon-Inducible T Cell Alpha Chemoattractant).
NCBI Gene ID #:
6373
NCBI Official Name:
chemokine (C-X-C motif) ligand 11
NCBI Official Symbol:
CXCL11
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O14625
Protein GI Number:
7674360
Purification:
Anti-hI-TAC specific antibody was purified by affinity chromatography employing immobilized hI-TAC matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
O14625
User NOte:
Centrifuge vial prior to opening.