LIGHT Antibody
| Code | Size | Price |
|---|
| PSI-XP-5215-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
LIGHT antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
LTg, TR2, CD258, HVEML, LIGHT, UNQ391/PRO726, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L
Application Note:
ELISA:
To detect hLIGHT by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hLIGHT.
Sandwich:
To detect hLIGHT by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human LIGHT (XP-5215Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.
Western Blot:
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hLIGHT by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hLIGHT.
Sandwich:
To detect hLIGHT by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human LIGHT (XP-5215Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.
Western Blot:
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT (human LIGHT).
NCBI Gene ID #:
8740
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 14
NCBI Official Symbol:
TNFSF14
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O43557
Protein GI Number:
313104028
Purification:
Anti-hLIGHT specific antibody was purified by affinity chromatography employing immobilized hLIGHT matrix.
Research Area:
Infectious Disease,Apoptosis,Chemokines & Cytokines,Herpes,Antibody Pairs
Swissprot #:
O43557
User NOte:
Centrifuge vial prior to opening.