MCP-4 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5224-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MCP-4 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
NCC1, CKb10, MCP-4, NCC-1, SCYL1, SCYA13, MCP4, NCC1, C-C motif chemokine 13, CK-beta-10
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (30.00 ng/mL), a concentration of 3.0 - 4.0 μg/mL of this antibody is required.
ELISA:
To detect hMCP-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCP-4.
Sandwich:
To detect hMCP-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MCP-4 (XP-5224Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4.
Western Blot:
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (30.00 ng/mL), a concentration of 3.0 - 4.0 μg/mL of this antibody is required.
ELISA:
To detect hMCP-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCP-4.
Sandwich:
To detect hMCP-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MCP-4 (XP-5224Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4.
Western Blot:
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-4 (human Macrophage/Monocyte chemotactic protein-4).
NCBI Gene ID #:
6357
NCBI Official Name:
chemokine (C-C motif) ligand 13
NCBI Official Symbol:
CCL13
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q99616
Protein GI Number:
3024128
Purification:
Anti-hMCP-4 specific antibody was purified by affinity chromatography employing immobilized hMCP-4 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
Q99616
User NOte:
Centrifuge vial prior to opening.