SDF-1a Antibody

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ProSci
Product Code: PSI-XP-5269Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5269Bt-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
SDF-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze

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To detect Human SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human SDF-1-alpha (XP-5269) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human SDF-1-alpha.
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To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect Human SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human SDF-1-alpha (XP-5269) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human SDF-1-alpha.
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Further Information

Additional Names:
IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12, SDF1A, SDF1B, Stromal cell-derived factor 1, C-X-C motif chemokine 12, SDF-1
Application Note:
ELISA:

Sandwich:
To detect hSDF-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human SDF-1α (XP-5269) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α.

Western Blot:
To detect hSDF-1-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-α (human SDF-1-α).
NCBI Gene ID #:
6387
NCBI Official Name:
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol:
CXCL12
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P48061
Protein GI Number:
1352728
Purification:
Anti-hSDF-1-α specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P48061
User NOte:
Centrifuge vial prior to opening.