Tarc Antibody
| Code | Size | Price |
|---|
| PSI-XP-5279-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
Tarc antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
TARC, ABCD-2, SCYA17, A-152E5.3, TARC, C-C motif chemokine 17, CC chemokine TARC,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTARC (100.00 ng/mL), a concentration of 20.0 - 30 μg/mL of this antibody is required.
ELISA:
To detect hTARC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTARC.
Sandwich:
To detect hTARC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TARC (XP-5279Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTARC.
Western Blot:
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hTARC (100.00 ng/mL), a concentration of 20.0 - 30 μg/mL of this antibody is required.
ELISA:
To detect hTARC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTARC.
Sandwich:
To detect hTARC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TARC (XP-5279Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTARC.
Western Blot:
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TARC displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. TARC binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTARC (human Thymus and Activation Regulated Chemokine).
NCBI Gene ID #:
6361
NCBI Official Name:
chemokine (C-C motif) ligand 17
NCBI Official Symbol:
CCL17
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q92583
Protein GI Number:
3024711
Purification:
Anti-hTARC specific antibody was purified by affinity chromatography employing immobilized hTARC matrix.
Research Area:
Chemokines & Cytokines,Immunology,Antibody Pairs, Functional Assays
Swissprot #:
Q92583
User NOte:
Centrifuge vial prior to opening.