TECK Antibody
| Code | Size | Price |
|---|
| PSI-XP-5280-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
TECK antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
TECK, Ckb15, SCYA25, TECK, C-C motif chemokine 25, Chemokine TECK,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0- 15.0 μg/mL of this antibody is required.
ELISA:
To detect hTECK by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK.
Sandwich:
To detect hTECK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TECK (XP-5280Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK.
Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0- 15.0 μg/mL of this antibody is required.
ELISA:
To detect hTECK by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK.
Sandwich:
To detect hTECK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TECK (XP-5280Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK.
Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine).
NCBI Gene ID #:
6370
NCBI Official Name:
chemokine (C-C motif) ligand 25
NCBI Official Symbol:
CCL25
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O15444
Protein GI Number:
212286066
Purification:
Anti-hTECK specific antibody was purified by affinity chromatography employing immobilized hTECK matrix.
Research Area:
Chemokines & Cytokines,Immunology,Antibody Pairs, Functional Assays
Swissprot #:
O15444
User NOte:
Centrifuge vial prior to opening.