VEGF Antibody

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ProSci
Product Code: PSI-XP-5291
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5291-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
VEGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t

Images

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To detect hVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human VEGF (XP-5291Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.
2 / 5
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 5
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 5
This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
5 / 5
This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
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To detect hVEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human VEGF (XP-5291Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended concentrations are 2.5 - 5.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

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Further Information

Additional Names:
VPF, VEGF, MVCD1, Vascular endothelial growth factor A, Vascular permeability factor, VEGF-A
Application Note:


Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hVEGF (10 ng/mL), a concentration of 0.05 - 0.1 μg/mL of this antibody is required.

ELISA:

To detect hVEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hVEGF.

Sandwich:

To detect hVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human VEGF (XP-5291Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.

Western Blot:
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVEGF (human Vascular Endothelial Cell Growth Factor).
NCBI Gene ID #:
7422
NCBI Official Name:
vascular endothelial growth factor A
NCBI Official Symbol:
VEGFA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P15692-4
Protein GI Number:
17380528
Purification:
Anti-hVEGF specific antibody was purified by affinity chromatography employing immobilized hVEGF matrix.
Research Area:
Signal Transduction,Growth Factor,Antibody Pairs
Swissprot #:
P15692-4
User NOte:
Centrifuge vial prior to opening.