Leptin Antibody
| Code | Size | Price |
|---|
| PSI-XP-5211Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Leptin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
ob, obese, Ob, Leptin, Obesity factor,
Application Note:
ELISA:
Sandwich:
To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine Leptin (XP-5211) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
Western Blot:
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine Leptin (XP-5211) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
Western Blot:
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLeptin (murine Leptin).
NCBI Gene ID #:
16846
NCBI Official Name:
leptin
NCBI Official Symbol:
Lep
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P41160
Protein GI Number:
730219
Purification:
Anti-mLeptin specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Signal Transduction,Growth Factor,Obesity,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P41160
User NOte:
Centrifuge vial prior to opening.