MIP-1a Antibody
| Code | Size | Price |
|---|
| PSI-XP-5234-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MIP-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
Mip1a, Scya3, G0S19-1, AI323804, MIP1-(a), LD78alpha, MIP-1alpha, MIP1-alpha, Mip1a, C-C motif chemokine 3, Heparin-binding chemotaxis protein, MIP-1-alpha
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-1-alpha (10 ng/mL), a concentration of 2.0 - 5.0 μg/mL of this antibody is required.
ELISA:
To detect mMIP-1-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1-alpha.
Sandwich:
To detect hMIP-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MIP-1α (XP-5234Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1α.
Western Blot:
To detect mMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-1-alpha (10 ng/mL), a concentration of 2.0 - 5.0 μg/mL of this antibody is required.
ELISA:
To detect mMIP-1-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1-alpha.
Sandwich:
To detect hMIP-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MIP-1α (XP-5234Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1α.
Western Blot:
To detect mMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1-alpha (murine Macrophage Inflammatory Protein-1-alpha).
NCBI Gene ID #:
20302
NCBI Official Name:
chemokine (C-C motif) ligand 3
NCBI Official Symbol:
Ccl3
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P10855
Protein GI Number:
127079
Purification:
Anti-mMIP-1-alpha specific antibody was purified by affinity chromatography employing immobilized mMIP-1-alpha matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P10855
User NOte:
Centrifuge vial prior to opening.