SCF Antibody
| Code | Size | Price |
|---|
| PSI-XP-5266-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
SCF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th
Documents
Further Information
Additional Names:
Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, blz, Kitlg, contrasted, Kitl, Slf, Kit ligand, Hematopoietic growth factor KL, MGF
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μg/mL of this antibody is required.
ELISA:
To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF.
Sandwich:
To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.
Western Blot:
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μg/mL of this antibody is required.
ELISA:
To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF.
Sandwich:
To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.
Western Blot:
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF).
NCBI Gene ID #:
17311
NCBI Official Name:
kit ligand
NCBI Official Symbol:
Kitl
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P20826
Protein GI Number:
134290
Purification:
Anti-mSCF specific antibody was purified by affinity chromatography employing immobilized mSCF matrix.
Research Area:
Signal Transduction,Immunology,Antibody Pairs, Functional Assays
Swissprot #:
P20826
User NOte:
Centrifuge vial prior to opening.