SCF Antibody
| Code | Size | Price |
|---|
| PSI-XP-5266Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
SCF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th
Documents
Further Information
Additional Names:
Gb, SF, Sl, Clo, Con, Mgf, SCF, SLF, blz, Kitlg, contrasted, Kitl, Slf, Kit ligand, Hematopoietic growth factor KL, MGF
Application Note:
ELISA:
Sandwich:
To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine SCF (XP-5266) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.
Western Blot:
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine SCF (XP-5266) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.
Western Blot:
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF).
NCBI Gene ID #:
17311
NCBI Official Name:
kit ligand
NCBI Official Symbol:
Kitl
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P20826
Protein GI Number:
134290
Purification:
Anti-mSCF specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Signal Transduction,Immunology,Antibody Pairs
Swissprot #:
P20826
User NOte:
Centrifuge vial prior to opening.