SF-20 Antibody
| Code | Size | Price |
|---|
| PSI-38-190-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
SF-20 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
Il25, Ly6elg, Il25, UPF0556 protein C19orf10 homolog, Interleukin-25, IL-25
Application Note:
ELISA:
Sandwich:
To detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine SF-20 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.
Western Blot:
To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect mSF-20 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine SF-20 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSF-20.
Western Blot:
To detect mSF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSF-20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
SF20, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSF-20. Murine SF-20 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
28106
NCBI Official Name:
DNA segment, Chr 17, Wayne State University 104, expressed
NCBI Official Symbol:
D17Wsu104e
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q9CPT4
Protein GI Number:
61221740
Research Area:
Antibody Pairs
Swissprot #:
Q9CPT4
User NOte:
Centrifuge vial prior to opening.