EGF Antibody
| Code | Size | Price |
|---|
| PSI-38-210-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
AntiEGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freez
Documents
Further Information
Additional Names:
Pro-epidermal growth factorEGF
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect Rat EGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Sandwich
To detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Western Blot:
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of Rat EGF (0.1 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect Rat EGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Sandwich
To detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat EGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
Western Blot:
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor is also a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Rat EGF specific antibody was purified by affinity chromatography employing immobilized Rat EGF matrix.
NCBI Gene ID #:
25313
NCBI Official Name:
epidermal growth factor
NCBI Official Symbol:
Egf
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P07522
Protein GI Number:
1352360
Research Area:
Growth Factor,Antibody Pairs, Functional Assays
Swissprot #:
P07522
User NOte:
Centrifuge vial prior to opening.