RANTES Antibody
| Code | Size | Price |
|---|
| PSI-XP-5258-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
RANTES antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
Scya5, Rantes, Scya5, C-C motif chemokine 5, SIS-delta,
Application Note:
ELISA:
To detect Rat Rantes by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat Rantes.
Sandwich:
To detect Rat RANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat RANTES (XP-5258Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES.
Western Blot:
To detect Rat Rantes by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Rantes is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat Rantes by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat Rantes.
Sandwich:
To detect Rat RANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat RANTES (XP-5258Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES.
Western Blot:
To detect Rat Rantes by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Rantes is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat Rantes.
NCBI Gene ID #:
81780
NCBI Official Name:
chemokine (C-C motif) ligand 5
NCBI Official Symbol:
Ccl5
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P50231
Protein GI Number:
1711393
Purification:
Anti-rat Rantes specific antibody was purified by affinity chromatography employing immobilized rat RANTES matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P50231
User NOte:
Centrifuge vial prior to opening.