Radixin Antibody

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ProSci
Product Code: PSI-45-137
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-137-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-137 (0.03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-137 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-137 (0.03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-137 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
RDX, radixin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3, HeLa, Jurkat, and A431. The observed molecular weight corresponds to earlier findings in literature (Magendantz et al, J Biol Chem. 1995 Oct 27;270(43):25324-7. PMID: 7592691). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 2.5ug/ml.
Background References:
  • Ivetic A, Florey O, Deka J, Haskard DO, Ager A, Ridley AJ. Mutagenesis of the ezrin-radixin-moesin binding domain of L-selectin tail affects shedding, microvillar positioning, and leukocyte tethering. J Biol Chem. 2004 Aug 6;279(32):33263-72. Epub 2004 Jun 3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QARDETKKTQND
NCBI Gene ID #:
5962
NCBI Official Name:
radixin
NCBI Official Symbol:
RDX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002897.1
Protein GI Number:
4506467
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
.
Swissprot #:
P35241