SLC7A5 Antibody

ProSci
Product Code: PSI-42-149
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-149-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-149 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

42-149 (0.3ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
SLC7A5, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5, 4F2LC, CD98, D16S469E, E16, LAT1, MPE16, hLAT1, 4F2 light chain, CD98 light chain, L-type amino acid transporter 1, integral membrane protein E16, large neutral amino acids transporter 1, large neutral amino acids transporter small subunit 1, sodium-independent neutral amino acid transporter LAT1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Spleen lysates (calculated MW of 55.0kDa according to NP_003477.4). Recommended concentration: 1-3ug/ml. Some minor background is detected and is blocked by the immunizing peptide. We call for caution when used for other assays than Western blot.
Background References:
  • Ohno Y, Suda K, Masuko K, Yagi H, Hashimoto Y, Masuko T. Production and characterization of highly tumor-specific rat monoclonal antibodies recognizing the extracellular domain of human L-type amino-acid transporter 1. Cancer Sci. 2008 May;99(5):1000-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-RHRKPELERPIKVN
NCBI Gene ID #:
8140
NCBI Official Name:
solute carrier family 7 (amino acid transporter light chain, L system), member 5
NCBI Official Symbol:
SLC7A5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003477.4
Protein GI Number:
71979932
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
Q01650