SOD2 Antibody

ProSci
Product Code: PSI-42-386
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-386-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-386 (0.1ug/ml) staining of HeLa, HepG2, HEK293 and NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-386 (0.1ug/ml) staining of HeLa, HepG2, HEK293 and NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
SOD2, superoxide dismutase 2, mitochondrial, RP1-56L9.2, IPOB, MNSOD, MVCD6, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase [Mn], mitochondrial
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 24kDa band observed in Human Brain (cerebellum) lysates and in Mouse and Rat Brain and Spinal Cord lysates, while approx 26kDa band was observed in lysates of cell lines HeLa, HepG2, HEK293 and NIH3T3 (calculated MW of 24.8kDa according to Human NP_000627.2, and 24.6kDa according to Mouse NP_038699.2). Recommended concentration: 0.01-0.1ug/ml. Primary incubation was 1 hour.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow, Zebrafish
Immunogen:
The immunogen for this antibody is: C-EAIKRDFGSFDK
NCBI Gene ID #:
6648
NCBI Official Name:
superoxide dismutase 2, mitochondrial
NCBI Official Symbol:
SOD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000627.2, NP_001019637.1
Protein GI Number:
67782305
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
NB: The immunizing peptide represents the acetylation site including K122 according to isoform A. This antibody is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1) . Reported variants represent identical protein: NP_000627.2; NP_001019637.1.
Swissprot #:
P04179