STRA6 Antibody

ProSci
Product Code: PSI-46-715
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-715-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-715 (1.7ug/ml) staining of 3T3-L1 cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-715 (1.7ug/ml) staining of 3T3-L1 cell lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
STRA6, stimulated by retinoic acid gene 6 homolog (mouse) , FLJ12541, MCOPS9, PP14296 , stimulated by retinoic acid gene 6 homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-90kDa band observed in Rat Testis lysates, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 73.8kDa according to Rat NP_001025095.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.

An approx. 90kDa band was also observed in 3T3-L1 adipocytes reduced by stra6 siRNA. The 90kDa is stained after retrovirally overexpressing Mouse Stra6 and not after overexpressing Human Stra6 in 3T3 preadipocytes (calculated MW of 73.8kDa according to Mouse NP_033317.2). Primary incubation was 3 hours.

Background References:
  • Kawaguchi R, Yu J, Honda J, Hu J, Whitelegge J, Ping P, Wiita P, Bok D, Sun H. A membrane receptor for retinol binding protein mediates cellular uptake of vitamin A. Science. 2007 Feb 9;315(5813):820-5. Epub 2007 Jan 25.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QAFRKAALTSAKAN
NCBI Gene ID #:
20897 (mouse); 363071 (rat);
NCBI Official Name:
stimulated by retinoic acid gene 6
NCBI Official Symbol:
STRA6
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_033317.2, NP_001025095.1
Protein GI Number:
6678171
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
O70491