IL-8 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5706-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IL-8 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&#
Documents
Further Information
Additional Names:
Interleukin 8, CXCL8, Interleukin-8, C-X-C motif chemokine 8, IL-8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-8 when used with our biotinylated antigen affinity purified anti-human IL-8 (XP-5199Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-8 when used with our biotinylated antigen affinity purified anti-human IL-8 (XP-5199Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.
Background:
IL-8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-8 as the immunizing antigen.
NCBI Gene ID #:
3576
NCBI Official Name:
interleukin 8
NCBI Official Symbol:
IL8
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P10145
Protein GI Number:
124359
Purification:
This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
Research Area:
Immunology,Chemokines & Cytokines
Swissprot #:
P10145
User NOte:
Centrifuge vial prior to opening.