SCF Antibody

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ProSci
Product Code: PSI-XP-5723-M
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5723-M-0.5mg0.5mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
SCF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4

Images

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This antibody stained CACO-2 cells and A-431cells. The primary antibody was incubated at 2.0 ug/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
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This antibody stained CACO-2 cells and A-431cells. The primary antibody was incubated at 2.0 ug/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Documents

Further Information

Additional Names:
KIT ligand, MGF, Stem cell factor precursor, Mast cell growth factor, SCF, Kit ligand, SF, FPH2, KL-1, Kitl, SHEP7
Application Note:
ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human SCF when used with our biotinylated antigen affinity purified anti-human SCF (XP-5264Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.

Western Blot:
To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 2.0-4.0 ng/lane, under non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hSCF as the immunizing antigen.
NCBI Gene ID #:
4254
NCBI Official Name:
KIT ligand
NCBI Official Symbol:
KITLG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P21583
Protein GI Number:
134289
Purification:
This IgG1K antibody was purified from ascites fluid on a Protein A affinity column.
Research Area:
Signal Transduction,Immunology
Swissprot #:
P21583
User NOte:
Centrifuge vial prior to opening.