TNF-a Antibody
| Code | Size | Price |
|---|
| PSI-XP-5711-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
TNF-α lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at
Documents
Further Information
Additional Names:
tumor necrosis factor alpha, TNFA, TNFSF2, Tumor necrosis factor, Cachectin, TNF-a,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.5 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human TNF-α when used with our biotinylated antigen affinity purified anti-human TNF-α (XP-5282Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.5 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human TNF-α when used with our biotinylated antigen affinity purified anti-human TNF-α (XP-5282Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Background:
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hTNF-alpha as the immunizing antigen.
NCBI Gene ID #:
7124
NCBI Official Name:
tumor necrosis factor
NCBI Official Symbol:
TNF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01375
Protein GI Number:
135934
Research Area:
Immunology,Chemokines & Cytokines,Signal Transduction,Growth Factor, Functional Assays
Swissprot #:
P01375
User NOte:
Centrifuge vial prior to opening.