IL-11 Antibody

ProSci
Product Code: PSI-XP-5707-M
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5707-M-0.5mg0.5mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
IL-11 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&

Images

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To a sandwich ELISA (assuming 100ul/well), a concentration of 8.0-9.0 ug/ml of this antibody will detect recombinant human IL-11 when used with ProSci's biotinylated antigen affinity purified anti-human IL-11 (XP-5164Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.
2 / 4
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
3 / 4
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
4 / 4
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue.  The primary antibody was incubated at 2.5 ug/ml overnight at 4°C.  This was followed by a fluorophore conjugated secondary antibody.  Optimal concentrations and conditions may vary.

To a sandwich ELISA (assuming 100ul/well), a concentration of 8.0-9.0 ug/ml of this antibody will detect recombinant human IL-11 when used with ProSci's biotinylated antigen affinity purified anti-human IL-11 (XP-5164Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue.  The primary antibody was incubated at 2.5 ug/ml overnight at 4°C.  This was followed by a fluorophore conjugated secondary antibody.  Optimal concentrations and conditions may vary.

Further Information

Additional Names:
Interleukin 11Interleukin-11, Adipogenesis inhibitory factor, IL-11, AGIF
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of human IL-11 (5.0 ng/mL), a concentration of 2.4 - 3.6 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL of this antibody will detect at least 0.2 ng/well of recombinant human IL-11 when used with our biotinylated antigen affinity purified anti-human IL-11(XP-5164Bt) as the detection antibody at a concentration of at least 2 μg/mL.

Western blot:

To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human IL-11 as the immunizing antigen.
NCBI Gene ID #:
3589
NCBI Official Name:
interleukin 11
NCBI Official Symbol:
IL11
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P20809
Protein GI Number:
124294
Purification:
This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.
Research Area:
Immunology,Chemokines & Cytokines,Neuroscience, Functional Assays
Swissprot #:
P20809
User NOte:
Centrifuge vial prior to opening.