ZYX Antibody

ProSci
Product Code: PSI-42-257
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-257-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-257 (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

42-257 (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ZYX, zyxin, ESP-2, HED-2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 85kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 61.3kDa according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Li B, Zhuang L, Trueb B, Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. The Journal of biological chemistry 2004 May 279 (19): 20401-10.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QQREKPRVQEKQH
NCBI Gene ID #:
7791
NCBI Official Name:
zyxin
NCBI Official Symbol:
ZYX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003452.1
Protein GI Number:
4508047
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription ,Signal Transduction ,Neuroscience
SPECIFICITY:
Reported variants represent identical protein: NP_003452.1, NP_001010972.1
Swissprot #:
Q15942