Glutathione Peroxidase 1 Antibody

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ProSci
Product Code: PSI-45-065
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-065-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-065 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-065 (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-065 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-065 (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
glutathione peroxidase 1 , HGNC:4553, GPX1, GSHPX1, MGC14399, MGC88245
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration: 3-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml. Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration: 20ug/ml.
Background References:
  • Kato S, Kato M, Abe Y, Matsumura T, Nishino T, Aoki M, Itoyama Y, Asayama K, Awaya A, Hirano A, Ohama E. Redox system expression in the motor neurons in amyotrophic lateral sclerosis (ALS): immunohistochemical studies on sporadic ALS, superoxide dismutase 1 (SOD1)-mutated familial ALS, and SOD1-mutated ALS animal models. Acta Neuropathol (Berl). 2005 Jun 28;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-REALPAPSDDATA
NCBI Gene ID #:
2876
NCBI Official Name:
glutathione peroxidase 1
NCBI Official Symbol:
GPX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000572.2
Protein GI Number:
41406084
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ,Signal Transduction
SPECIFICITY:
This antibody is expected to recognise isoform 1 ( NP_000572.2) only.
Swissprot #:
P07203