PPPDE1 Antibody

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ProSci
Product Code: PSI-42-434
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-434-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PPPDE1 with C-terminal MYC tag probed with 42-434 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/5000) in lane C. Mock-transfected HEK293 probed with 42-434 (1mg/ml) in Lane B. Prima
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PPPDE1 with C-terminal MYC tag probed with 42-434 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/5000) in lane C. Mock-transfected HEK293 probed with 42-434 (1mg/ml) in Lane B. Prima

Documents

Further Information

Additional Names:
PPPDE1, PPPDE peptidase domain containing 1, C1orf121, CGI-146, FAM152A, FLJ21998, PNAS-4, family with sequence similarity 152, member A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing full-length Human PPPDE1(myc and DYKDDDDK tagged), a band of approx. 30kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Skin. Recommended concentration: 10ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-ELGKEYKGNAYH
NCBI Gene ID #:
51029
NCBI Official Name:
desumoylating isopeptidase 2
NCBI Official Symbol:
DESI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057160.2
Protein GI Number:
38708309
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Apoptosis
SPECIFICITY:
.
Swissprot #:
Q9BSY9