TNF-a Antibody
| Code | Size | Price |
|---|
| PSI-XP-5283Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
TNF-a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, TNF-a
Application Note:
ELISA:
Sandwich:
To detect hTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TNF-α (XP-5283) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α.
Western Blot:
To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TNF-α (XP-5283) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α.
Western Blot:
To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTNF-alpha (human hTNF-alpha).
NCBI Gene ID #:
7124
NCBI Official Name:
tumor necrosis factor
NCBI Official Symbol:
TNF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01375
Protein GI Number:
135934
Purification:
Anti-hTNF-alpha specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Immunology,Chemokines & Cytokines,Signal Transduction,Growth Factor,Antibody Pairs
Swissprot #:
P01375
User NOte:
Centrifuge vial prior to opening.