MCP-3 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5223Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
MCP-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
fic, marc, mcp3, MCP-3, Scya7, Fic, Mcp3, C-C motif chemokine 7, Monocyte chemoattractant protein 3
Application Note:
ELISA:
Sandwich:
To detect mMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MCP-3 (XP-5223) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
Western Blot:
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect mMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MCP-3 (XP-5223) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
Western Blot:
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mMCP-3 (murine MCP-3).
NCBI Gene ID #:
20306
NCBI Official Name:
chemokine (C-C motif) ligand 7
NCBI Official Symbol:
Ccl7
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q03366
Protein GI Number:
417192
Purification:
Anti-mMCP-3 specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
Q03366
User NOte:
Centrifuge vial prior to opening.