MIP-1B Antibody
| Code | Size | Price |
|---|
| PSI-XP-5235-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MIP-1B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
ACT2, G-26, HC21, LAG1, LAG-1, MIP1B, SCYA2, SCYA4, MIP1B1, AT744.1, MIP-1-beta, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/mL), a concentration of 1.75 - 3.0 μg/mL of this antibody is required.
ELISA:
To detect hMIP-1-beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1-beta.
Sandwich:
To detect hMIP-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-1β (XP-5235Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1β.
Western Blot:
To detect hMIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/mL), a concentration of 1.75 - 3.0 μg/mL of this antibody is required.
ELISA:
To detect hMIP-1-beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1-beta.
Sandwich:
To detect hMIP-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-1β (XP-5235Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1β.
Western Blot:
To detect hMIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1-beta.
NCBI Gene ID #:
6351
NCBI Official Name:
chemokine (C-C motif) ligand 4
NCBI Official Symbol:
CCL4
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P13236
Protein GI Number:
127080
Purification:
Anti-hMIP-1-beta specific antibody was purified by affinity chromatography employing immobilized hMIP-1-beta matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P13236
User NOte:
Centrifuge vial prior to opening.