GCH1 Antibody
| Code | Size | Price |
|---|
| PSI-46-847-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
GCH1 , GTP cyclohydrolase 1 (dopa-responsive dystonia) , DYT5, GCH, GTP-CH-1, GTPCH1, GTP cyclohydrolase 1, guanosine 5'-triphosphate cyclohydrolase I
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 37kDa band observed in Human Tonsil lysates. Customer?s experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9kDa according to NP_000152.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum) and Small Intestine. Recommended concentration: 5ug/ml.
Background References:
- Tegeder I, Costigan M, Griffin RS, Abele A, Belfer I, Schmidt H, Ehnert C, Nejim J, Marian C, Scholz J, Wu T, Allchorne A, Diatchenko L, Binshtok AM, Goldman D, Adolph J, Sama S, Atlas SJ, Carlezon WA, Parsegian A, Lotsch J, Fillingim RB, Maixner W, Geiss GTP cyclohydrolase and tetrahydrobiopterin regulate pain sensitivity and persistence. Nat Med. 2006 Nov;12(11):1269-77. Epub 2006 Oct 22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-GKVHIGYLPNKQ
NCBI Gene ID #:
2643
NCBI Official Name:
GTP cyclohydrolase 1
NCBI Official Symbol:
GCH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000152.1 , NP_001019195.1 , NP_001019241.1 , NP_001019242.1
Protein GI Number:
4503949
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience
SPECIFICITY:
This antibody is expected to recognize all four isoforms (NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1)
Swissprot #:
P30793