IRAK4 Antibody

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ProSci
Product Code: PSI-45-074
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-074-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-074 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-074 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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45-074 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-074 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Documents

Further Information

Additional Names:
IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in lysates of cell line HeLa and approx 55kDa in lysates of cell line Jurkat (calculated MW of 51.5kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2?g/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75?g/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10?g/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Lye E, Mirtsos C, Suzuki N, Suzuki S, Yeh WC. The role of interleukin 1 receptor-associated kinase-4 (IRAK-4) kinase activity in IRAK-4-mediated signaling. J Biol Chem. 2004 Sep 24;279(39):40653-8. Epub 2004 Jul 29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: NKPITPSTYVRC
NCBI Gene ID #:
51135
NCBI Official Name:
interleukin-1 receptor-associated kinase 4
NCBI Official Symbol:
IRAK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057207.2, NP_001107654.1
Protein GI Number:
7705841
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
.
Swissprot #:
Q9NWZ3