Midkine Antibody
| Code | Size | Price |
|---|
| PSI-XP-5230-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Midkine antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freez
Documents
Further Information
Additional Names:
MK, ARAP, NEGF2, MK1, Midkine, Amphiregulin-associated protein, MK
Application Note:
ELISA:
To detect hMidkine by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMidkine.
Sandwich:
To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Midkine (XP-5230Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.
Western Blot:
To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMidkine by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMidkine.
Sandwich:
To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Midkine (XP-5230Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.
Western Blot:
To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMidkine.
NCBI Gene ID #:
4192
NCBI Official Name:
midkine (neurite growth-promoting factor 2)
NCBI Official Symbol:
MDK
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P21741
Protein GI Number:
127116
Purification:
Anti-hMidkine specific antibody was purified by affinity chromatography employing immobilized hMidkine matrix.
Research Area:
Neuroscience,Antibody Pairs
Swissprot #:
P21741
User NOte:
Centrifuge vial prior to opening.