Nanog Antibody
| Code | Size | Price |
|---|
| PSI-38-104-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Nanog antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog
Application Note:
ELISA:
Sandwich:
To detect hNanog by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required.
Western Blot:
To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hNanog by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required.
Western Blot:
To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Human Nanog specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
79923
NCBI Official Name:
Nanog homeobox
NCBI Official Symbol:
NANOG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9H9S0
Protein GI Number:
118573073
Purification:
Nanog antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Cancer,Antibody Pairs
Swissprot #:
Q9H9S0
User NOte:
Centrifuge vial prior to opening.