IL-2 Antibody
| Code | Size | Price |
|---|
| PSI-38-204-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IL-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
Il-2, Interleukin-2, T-cell growth factor, IL-2
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-2 (1.0 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect Rat IL-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Sandwich
To detect Rat IL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat IL-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Western Blot:
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-2 (1.0 ng/mL), a concentration of < 0.1 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect Rat IL-2 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Sandwich
To detect Rat IL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat IL-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Western Blot:
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Rat IL-2 specific antibody was purified by affinity chromatography employing immobilized Rat IL-2 matrix.
NCBI Gene ID #:
116562
NCBI Official Name:
interleukin 2
NCBI Official Symbol:
Il2
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P17108
Protein GI Number:
124328
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P17108
User NOte:
Centrifuge vial prior to opening.