MAPK3 Antibody

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ProSci
Product Code: PSI-45-087
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-087-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-087 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-087 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MAPK3, ERK1, mitogen-activated protein kinase 3, HGNC:6877, P44ERK1, P44MAPK, PRKM3, protein kinase, mitogen-activated 3 (MAP kinase 3 p44), HS44KDAP, HUMKER1A, MGC20180, OTTHUMP00000174538, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 45kDa band observed in HEK293 lysates (calculated MW of 43.1kDa according to NP_002737.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Background References:
  • Wang PY, Weng J, Anderson RG. OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation. Science. 2005 Mar 4;307(5714):1472-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: GGEPRRTEGVGP-C
NCBI Gene ID #:
5595
NCBI Official Name:
mitogen-activated protein kinase 3
NCBI Official Symbol:
MAPK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002737.2, NP_001035145.1, NP_001103361.1
Protein GI Number:
38257141
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
No cross-reactivity expected with ERK2
Swissprot #:
P27361