RANTES Antibody

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ProSci
Product Code: PSI-XP-5259
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5259-0.1mg0.1mg£524.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
RANTES antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze

Images

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To detect mRANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine RANTES (XP-5259Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRANTES.
2 / 4
To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 4
To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 4
This antibody stained colchicine injected mouse brain (somatosensory cortex) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
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To detect mRANTES by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine RANTES (XP-5259Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRANTES.
To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected mouse brain (somatosensory cortex) tissue. The primary antibody was incubated at 0.5 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

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Further Information

Additional Names:
SISd, Scya5, RANTES, TCP228, MuRantes, C-C motif chemokine 5,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/mL), a concentration of 6.0 - 8.0 μg/mL of this antibody is required.

ELISA:

To detect mRANTES by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mRANTES.

Sandwich:

To detect mRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine RANTES (XP-5259Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRANTES.

Western Blot:
To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRANTES (murine RANTES).
NCBI Gene ID #:
20304
NCBI Official Name:
chemokine (C-C motif) ligand 5
NCBI Official Symbol:
Ccl5
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P30882
Protein GI Number:
548926
Purification:
Anti-mRANTES specific antibody was purified by affinity chromatography employing immobilized mRANTES matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P30882
User NOte:
Centrifuge vial prior to opening.