PDGF-BB Antibody

Contact us
ProSci
Product Code: PSI-XP-5255Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5255Bt-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
PDGF-BB antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freez

Images

1 / 3
To detect hPDGF-BB by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human PDGF-BB (XP-5255) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hPDGF-BB.
2 / 3
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 3
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
❮❯

To detect hPDGF-BB by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human PDGF-BB (XP-5255) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hPDGF-BB.
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Documents

Further Information

Additional Names:
SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
Application Note:
ELISA:

Sandwich:
To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human PDGF-BB (XP-5255) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.

Western Blot:

To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor-BB).
NCBI Gene ID #:
5155
NCBI Official Name:
platelet-derived growth factor beta polypeptide
NCBI Official Symbol:
PDGFB
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01127
Protein GI Number:
129724
Purification:
Anti-hPDGF-BB specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Growth Factor,Antibody Pairs
Swissprot #:
P01127
User NOte:
Centrifuge vial prior to opening.