DCTN3 Antibody

ProSci
Product Code: PSI-42-682
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-682-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-682 (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-682 (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
DCTN3, dynactin 3 (p22), DCTN-22, DCTN22, MGC111190, dynactin 3, dynactin 3, isoform 1, dynactin light chain
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 20kDa band observed in Human Kidney, Skeletal Muscle and Pancreas lysates (calculated MW of 19.5kDa according to NP_077324.1). In addition, a 25kDa band is observed in both Mouse and Rat Skeletal Muscle lysates accompanied by an 18kDa band in Mouse Skeletal Msuscle. In Mouse the protein is known as p24. The 18kDa band could reprsent a smaller isoform. Recommended concentration: 0.03-0.1ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QVALGNISSKRE
NCBI Gene ID #:
11258
NCBI Official Name:
dynactin 3 (p22)
NCBI Official Symbol:
DCTN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009165.1, NP_077324.1
Protein GI Number:
6005745
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Cell Cycle
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Swissprot #:
O75935