RELMb Antibody

ProSci
Product Code: PSI-38-106
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-106-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
RELMb antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

Images

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To detect Murine RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine RELM-beta (38-102) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELM-beta.
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This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4˚C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.

To detect Murine RELM-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine RELM-beta (38-102) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELM-beta.
This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4˚C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
Xcp3, Fizz2, Relmb, RELMbeta, 9030012B21Rik, Resistin-like beta, Cysteine-rich secreted protein A12-beta,
Application Note:
ELISA:
Indirect:
To detect mRELMb by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMb.
Sandwich:
To detect mRELMb by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required.

Western Blot:
To detect mRELMb by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMb is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
RELM beta, is a secreted protein, a probable hormone, expressed only in the gastrointestinal tract, particularly the colon. The biological functions of RELM beta and its molecular targets, are not fully known but, it has been suggested that it plays a regulatory role during inflammation and may also act to establish links among adipose tissue, the intestine and the liver. Interestingly the molecular structure of RELM beta is highly homologous to that of the adipose-derived cytokine Resistin and RELM alpha.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMb. Murine RELMb specific antibody was purified by affinity chromatography employing immobilized RELMb matrix.
NCBI Gene ID #:
57263
NCBI Official Name:
resistin like beta
NCBI Official Symbol:
Retnlb
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q99P86
Protein GI Number:
17380313
Purification:
RELMb antibody was purified by affinity chromatography employing immobilized RELMb matrix.
Research Area:
Signal Transduction,Cancer,Antibody Pairs
Swissprot #:
Q99P86
User NOte:
Centrifuge vial prior to opening.