PF-4 Antibody

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ProSci
Product Code: PSI-XP-5256
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5256-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
PF-4 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t

Images

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To detect hPF-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human PF-4 (XP-5256Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4.
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To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
5 / 6
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
6 / 6
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
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To detect hPF-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human PF-4 (XP-5256Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4.
To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1.0 mg/mL - 2.0 mg/mL with an overnight incubation at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Documents

Further Information

Additional Names:
PF-4, CXCL4, SCYB4, Platelet factor 4, C-X-C motif chemokine 4, PF-4
Application Note:


ELISA:

To detect hPF-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPF-4.

Sandwich:

To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human PF-4 (XP-5256Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4.

Western Blot:

To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Platelet factor 4 (PF-4) is a 70 amino acid protein that is released from the alpha granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF-4 probably has a role in inflammation and wound repair.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4).
NCBI Gene ID #:
5196
NCBI Official Name:
platelet factor 4
NCBI Official Symbol:
PF4
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P02776
Protein GI Number:
130304
Purification:
Anti-hPF-4 specific antibody was purified by affinity chromatography employing immobilized hPF-4 matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P02776
User NOte:
Centrifuge vial prior to opening.