RANGAP1 Antibody

ProSci
Product Code: PSI-45-135
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-135-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-135 staining (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
2 / 2

45-135 staining (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
RANGAP1, KIAA1835, Ran GTPase activating protein 1, Fug1, MGC20266, SD, OTTHUMP00000198756, OTTHUMP00000198757, segregation distorter homolog, segregation distortion
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in 3T3 lysates (predicted MW of 68kDa according to NP_002874). Recommended for use at 0.1-0.5ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration: 2-4ug/ml.
Background References:
  • Bischoff FR, Krebber H, Kempf T, Hermes I, Ponstingl H. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc Natl Acad Sci USA. 1995 Feb 28;92(5):1749-53.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: ASEDIAKLAETLAK-C
NCBI Gene ID #:
5905
NCBI Official Name:
Ran GTPase activating protein 1
NCBI Official Symbol:
RANGAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002874.1
Protein GI Number:
4506411
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.
Swissprot #:
P46060