DAP3 Antibody

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ProSci
Product Code: PSI-45-046
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-046-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-046 (0.3ug/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
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45-046 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
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45-046 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
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45-046 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
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45-046 (0.3ug/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
45-046 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-046 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
45-046 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)

Documents

Further Information

Additional Names:
DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1ug/ml. Primary incubated 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10ug/ml.
Background References:
  • Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. J Biol Chem. 1995 Nov 17;270(46):27932-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: NPSLLERHCAYL
NCBI Gene ID #:
7818
NCBI Official Name:
death associated protein 3
NCBI Official Symbol:
DAP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_387506.1, NP_001186779.1, NP_001186780.1
Protein GI Number:
16905526
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
Swissprot #:
P51398