PIGF Antibody

ProSci
Product Code: PSI-29-291
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-29-291-100ul100ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PIGF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Further Information

Additional Names:
PIGF, MGC32646, MGC33136,
Application Note:
PIGF antibody can be used for detection of PIGF by ELISA at 1:12500. PIGF antibody can be used for detection of PIGF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PIGF is a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. PIGF and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. This gene encodes a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described.
Background References:
  • Mohammed, K.A., Am. J. Physiol. Lung Cell Mol. Physiol. 292 (2), L559-L566 (2007).
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIGF.
NCBI Gene ID #:
5281
NCBI Official Name:
phosphatidylinositol glycan anchor biosynthesis, class F
NCBI Official Symbol:
PIGF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
NP_002634
Protein GI Number:
4505797
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Membrane ,RNA Binding ,Signal Transduction
Swissprot #:
Q07326
User NOte:
Optimal dilutions for each application to be determined by the researcher.