APBB1 Antibody

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ProSci
Product Code: PSI-45-261
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-261-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-261 (0.1ug/ml) staining of NIH/3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-261 (0.1ug/ml) staining of NIH/3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
APBB1, FE65, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), MGC:9072, RIR , adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of NIH/3T3 (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273(32):20128-33.; PMID: 9685356). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Background References:
  • Hoe HS, Magill LA, Guenette S, Fu Z, Vicini S, Rebeck GW. FE65 Interaction with the apoE receptor ApoEr2. J Biol Chem. 2006 Apr 25;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-GSLKPKRLGAHTP
NCBI Gene ID #:
322
NCBI Official Name:
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
NCBI Official Symbol:
APBB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001155.1, NP_663722.1
Protein GI Number:
4502131
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).
Swissprot #:
O00213