ATXN1 Antibody

ProSci
Product Code: PSI-42-358
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-358-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
ATXN1, ataxin 1, ATX1, D6S504E, SCA1, OTTHUMP00000016065, ataxin-1, spinocerebellar ataxia type 1 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 75kDa and 26kDa in Mouse Fetal Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 84.7kDa according to NP_001186234.1 and 83.8kDa according to NP_033150.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TLKNLKNGSVKKGQ
NCBI Gene ID #:
6310
NCBI Official Name:
ataxin 1
NCBI Official Symbol:
ATXN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000323.2
Protein GI Number:
51479158
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
Reported variants represent identical protein: NP_001121636.1, NP_000323.2
Swissprot #:
P54253