BAFF (Buffy-2) Monoclonal Antibody

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ProSci
Product Code: PSI-XA-1012
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XA-1012-0.1mg0.1mg£686.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: Rat IgG2a
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
BAFF antibody can be stored at 4˚C, stable for one year.

Images

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The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-2) recognizes the TNF homology domain. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.
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The immunogen is recombinant human soluble BAFF, amino acids 83-285. Anti-BAFF (MAb Buffy-2) recognizes the TNF homology domain. Schematic structure of human soluble BAFF (rec.) (aa 83-285) as seen in this image.

Documents

Further Information

Application Note:
BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of MAb to BAFF (Buffy-2) (FITC). After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..
Background References:
  • Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002).
  • Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004).
  • Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004).
  • BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285).
NCBI Gene ID #:
10673
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 13b
NCBI Official Symbol:
TNFSF13B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Cytokine,Cancer
Swissprot #:
Q9Y275
User NOte:
Optimal dilutions for each application to be determined by the researcher.