BIRC5 Antibody

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ProSci
Product Code: PSI-45-023
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-023-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-023 (0.01ug/ml) staining of Molt-4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-023 (0.01ug/ml) staining of Molt-4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
BIRC5, survivin, API4, EPR-1, baculoviral IAP repeat-containing 5 (survivin), apoptosis inhibitor 4, baculoviral IAP repeat-containing 5, baculoviral IAP repeat-containing protein 5, survivin variant 3 alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 18kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kDa according to NP_001159.2 and 18.6kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5ug/ml.
Background References:
  • Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC. Control of apoptosis and mitotic spindle checkpoint by survivin. Nature. 1998 Dec 10;396(6711):580-4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CVRRAIEQLAAMD
NCBI Gene ID #:
332
NCBI Official Name:
baculoviral IAP repeat containing 5
NCBI Official Symbol:
BIRC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001159.2, NP_001012271.1
Protein GI Number:
59859878
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis
SPECIFICITY:
This antibody is expected to recognize the reported isoforms 1 (NP_001159.2) and isoform 3 (NP_001012271.1).
Swissprot #:
O15392