CARD6 Antibody

ProSci
Product Code: PSI-45-361
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-361-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-361 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-361 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
CARD6, caspase recruitment domain family, member 6, caspase recruitment domain protein 6, CINCIN1, CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10ug/ml.
Background References:
  • Stehlik C, Hayashi H, Pio F, Godzik A, Reed JC. CARD6 is a modulator of NF-kB activation by Nod1- and Cardiak-mediated pathways. J Biol Chem. 2003 May 29
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: ATESTPSEIIERER-C
NCBI Gene ID #:
84674
NCBI Official Name:
caspase recruitment domain family, member 6
NCBI Official Symbol:
CARD6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115976.2
Protein GI Number:
16554564
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9BX69